9/10/2023 0 Comments India ink staining whole blood![]() ![]() Smears that are too thick will most likely wash off the slide regardless of the fixation method. If your slide is wet and fix it in methanol, it will most likely wash off the slide. If your slide is wet and you heat fix it, the bacteria will boil and the cellular morphology will be lost. Do this consistently on the same end of the slide to help orient your slide.īe patient and take the time to let your slide air dry before proceeding with adhering it to the slide. Be sure to label the far edge of the slide. It is very easy to get confused which side of the slide your smear is on. You have lots of room on your slide use it! It helps to initially draw a circle on the bottom of the slide so you know where to look for your smear. You are striving for a light suspension of cells that will leave a faint cloudy deposit on your slide. Dispose of your completed slides in the disinfectant bucket at your bench. Heat or methanol fixation is not guaranteed to kill the organism. The loop is very flexible and it is easy to zing off a loop-full of organisms. Materialsīe careful of aerosols when transferring bacteria from your loop to the slide. It will undoubtedly take you several tries before you are successful. While the goals are the same for both, evenly and lightly dispersed cells firmly adhered to the slide surface, the techniques are slightly different. You will be preparing slides for staining from both broth and agar surfaces. The cells typically shrink in size and will exhibit some changes in shape and extra-cellular matrices. All procedures that attach the bacteria to the slide result in some morphological changes. The bacteria need to be firmly attached to the slide so they are not washed off during the staining procedures.Large blobs of cells also do not stain properly and could yield erroneous results from the improper staining. If there are too many bacteria on the slide they will form a big glob and you will not be able to see the morphology of the individual cells. The bacteria must be evenly and lightly dispersed.There are two important things to consider when preparing a slide for staining: You must firmly attach your bacteria to a glass slide before you can stain them. Diagnostic Microbiology & Infectious Disease, 55, 275-278.Not only are most bacteria very small, they are also very clear and difficult to view under a microscope without first staining. Differentiation Of Tuberculous Meningitis From Acute Bacterial Meningitis Using Simple Clinical And Laboratory Parameters. Strategies To Reduce Mortality And Morbidity Due To Aids-Related Cryptococcal Meningitis In Latin America. Critical Care And Resuscitation, 2, 42-54. Microbiology An Introduction, San Francisco, Pearson. Diagnostic Microbiology, Misouri: Elsevier. Manual Of Clinical Microbiology, Washington Dc: Asm Press. ![]() Clinical Utility Of The Cryptococcal Antigen Lateral Flow Assay In A Diagnostic Mycology Laboratory. C., Judd, D., Sleiman, S., Marriott, D., Olma, T. Principles And Practice Of Infectious Disease, Usa: Churchill Livingstone. Texbook Of Diagnostic Microbiology: Elsevier. Long-Term Outcome Of Aids-Associated Cryptococcosis In The Era Of Combination Antiretroviral Therapy. N., Boibieuxf, A., Muriel Alvarezg, Dellamonicah, P. Lortholarya, O., Poizatc, G., Zellerd, V.Fundamental Of Diagnostic Mycology, Philadelphia, Wb Saunders Company. An Insight Into The Ligand–Receptor Interactions Involved Inthe Translocation Of Pathogens Across Blood–Brain Barrier Fems Immunol Med Microbiol, 63, 297-318. India Ink Cryptococcal Meningitis HIV-AIDS The patient was diagnosed with Cryptococcal meningitis by indian ink staining, and immediately given anti-fungal theraphy. neoformans is the most common cause of Cryptococcosis in patients with HIV-AIDS. India ink stain showed encapsulated yeasts.Ĭryptococcus sp is the only encapsulated yeast, while C. blood glucose 293 mg / dl), elevated protein concentration (137.1 mg / dl), and polymorphonuclear pleocytosis. Laboratory finding included a clowdy/turbid Cerebrospinal fluid (CSF), low glucose level (CSF glucose 43 mg / dl vs. Routine blood was normal, 60 CD4 cells/mm3. On physical examination, he was compos mentis, meningeal’s stimuli signs (+), where as on examination of craniales nerves, motor and sensibility was in a normal range. The cryptococcal polysaccharide antigen test for diagnosis of meningitis is rapid but relatively expensive while culture is time consuming.Ī 47-year man was admitted to hospital with a headache, fever, nausea, and vomiting and a HIV history for the last 6 months. A rapid diagnosis plays an important role to ensure a prompt therapy of the disease. This infection causes increased morbidity and mortality in patients with HIV-AIDS. Cryptococcal meningitis incidence has increased along with an increase in incidence of HIV-AIDS. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |